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MIL-V-82466(OS)
4.4.4 Tropical environment.  The VAND System, including applicable
external connections, shall be placed in a mold chamber in which are
maintained a temperature of 86 degrees F plus or minus 5 degrees F and
a relative humidity of 90 percent plus or minus 5 percent, and the
system shall then be sprayed with a suspension of mixed spores.  These
conditions shall be maintained for twenty-eight days.  At the conclusion
of this period, the VAND System shall be allowed to return to room
temperature before its performance is checked.  At least five groups of
fungi shall be used in the system's inoculation.  These five groups are
listed below, and one type of fungus from each group shall be used. In
preparing the spore suspension, distilled water (having a pH value
between 5.8 and 7.2 degrees F) shall be prepared by sterilization
in convenient containers (approximately 100 milliliters each). Approxi-
mately 10 milliliters of the solution shall then be introduced directly
into a stock culture of one fungus and shaken vigorously in order that
a well sporulated suspension will result without disturbing the agar.
This process shall be repeated for each type fungus.  The separate spore
suspensions from the types of fungi shall then be mixed together in an
atomizer to provide a composite suspension.  Actively growing cultures
in ripe fruiting condition shall be used for each inoculation and under
no condition will the inoculum be kept for more than an 8 hour period.
Chaetomium globosum USDA 1042.4, ATCC 6205, or
Group I
Myrothecium verrucaria USDA 1334.2, ATCC 9095.
Group II
Rhizopus nigricans S.N. 32, ATCC 10404, or
Aspergillus niger USDA TC215-4247, ATCC 6275.
Group III
Aspergillus flavus AMC No. 26, ATCC 10836, or
Aspergillus terreus PQMD 82J, ATCC 10690.
Group IV
Penicillium luteum USDA 1336.1, ATCC 9776
Penicillium sp. USDA 1336.2, ATCC 9112, or
Penicillium citrinum ATCC 9849.
Group V
Memnoniella echinata AMC No. 37, ATCC 9597, or
Fusarium moniliforme USDA 1004.1, ATCC 10052.
4.4.5 Functional tests.  At the conclusion of the tests specified in
4.4.1.5 and 4.4.2.2 above, functional and other tests, as applicable,
shall be conducted on the system to determine freedom from operational
malfunction and compliance with all requirements of Section 3.
4.4.6 Waiver of tests.  Tests specified in 4.4.1.5 and 4.4.2.2 above
may be waived by the procuring agency for Level C packs only, provided
the contractor guarantees safe delivery to the using activity.
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