 |
|||
|
|
|||
| ||||||||||
|
|  MIL-R-51446(EA)
Weigh the CR and the internal standard to the nearest 0.1 mg into a 10-ml
volumetric flask. Dissolve and dilute to the volume mark with ethanol.
Saturate the chromatography column three times with 20-microliter portions of
the solution. Inject a fourth 20-microliter portion of the solution and
measure the resulting areas for the CR and the internal standard. Calculate
the response factor for CR, corrected for purity, as follows:
where: A =
Area per unit weight of the CR of known purity,
B=
Percent purity of the internal standard,
C=
Area per unit weight of the internal standard, and
D=
Percent purity of the CR.
(c) Procedure. Weigh to the nearest milligram approximately 0.5
g each of the specimen and the internal standard into a 10-ml volumetric flask.
Dissolve and dilute to the volume mark with ethanol. Saturate the chromato-
graphy column three times with 20-microliter portions of the solution. Inject a
fourth 20-microliter portion of the solution and measure the resulting areas.
(Using conditions in table I, 1,10-dibromodecane will elute in approximately 19
minutes at 173C and the specimen will elute in approximately 21 minutes at
185C). Calculate the percent by weight CR as follows:
Area produced by the specimen solution,
where: E =
F=
Weight in grams of the internal standard,
G=
Percent purity" of the internal standard,
H=
Area produced by the internal standard solution,
R=
Response factor calculated in (b), and
W=
Weight in grams of specimen.
4.2.3.4 Eye irritancy. Determine eye irritancy of the specimen in accord-
ance with 16 CFR 1500.42, Test for Eye Irritants, with the following modifica-
tions:
(a) For paragraph (a)(1) sentence 8, 0.1 ml of a solution of 1
percent by weight specimen in propylene glycol/water (80/20) is to be used.
(b) For paragraph (b)(1), substitute the following: the animal
shall be considered as exhibiting a positive reaction if the test substance
produces more than a mild reddening of the eyelid margins and slight injection
of the blood vessels of the conjunctival and nictitating membrane.
6
|
|
Privacy Statement - Press Release - Copyright Information. - Contact Us |
Click
here for thousands of PDF manuals